Measuring Allodynia and Hyperalgesia

The Von Frey filament test is used to measure allodynia and hyperalgesia and is a more direct way to measure visceral pain and hyperalgesia. Mice were individually housed and acclimatized to the modular animal enclosures (Stoelting, 57823) at least 3 days before the experiment. Tests were performed using Semmes–Weinstein Von Frey filaments (Stoelting, 58011) as previously described [70 (link), 71 (link)]. Filaments were applied to the abdomen or hind-paw ten times, the filament evoking a withdrawal response in at least 50% of applications was considered the threshold force (g).

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Bashir S.T., Redden C.R., Raj K., Arcanjo R.B., Stasiak S., Li Q., Steelman A.J, & Nowak R.A. (2023). Endometriosis leads to central nervous system-wide glial activation in a mouse model of endometriosis. Journal of Neuroinflammation, 20, 59.

Publication 2023

Abdomen Allodynia Animal Filament Hyperalgesia Mice Visceral pain

Corresponding Organization :

Other organizations : University of Illinois Urbana-Champaign

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Variable analysis

independent variables

Body region (abdomen, hind-paw)

dependent variables

Threshold force (g) required to elicit a withdrawal response in at least 50% of applications

control variables

Individual housing of mice
Acclimatization period of at least 3 days before the experiment
Use of Semmes–Weinstein Von Frey filaments

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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