EVL (RAD001, 99.69%) was obtained from Selleck Chemicals LLC (TX, USA). ITC and AMB were from Macklin (Shanghai, China). VRC and POS were from Solarbio Biotechnology (Beijing, China). RPMI-1640 was from Gen-view Scientific Inc. (FL, USA). All reagents were prepared as per Clinical and Laboratory Standards Institute M38-A2 guidelines (CLSI, 2008).
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