Quantifying IL-6 Levels in OP9 Cells

To determine levels of IL-6 in OP9 cells, samples were analyzed using a commercially available mouse IL-6 ELISA Kit, (RAB0308, Sigma-Aldrich, Tokyo, Japan). At the end of the cultivation process, cell culture supernatants were collected and reacted with the first and secondary antibodies, streptavidin-HRP, and detection solution according to the manufacture’s instruction. The reaction was stopped using the stop solution (from the ELISA kits) and absorbance was read at 450 nm using a microplate reader (SH-9000Lab, Hitachi, Tokyo, Japan) [23 (link)].

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Xiao L, & Miwa N. (2021). Hydrogen Nano-Bubble Water Suppresses ROS Generation, Adipogenesis, and Interleukin-6 Secretion in Hydrogen-Peroxide- or PMA-Stimulated Adipocytes and Three-Dimensional Subcutaneous Adipose Equivalents. Cells, 10(3), 626.

Publication 2021

mouse IL-6 ELISA Kit SH-9000Lab

Antibodies Cell culture Cells Elisa Mouse il 6 Streptavidin

Corresponding Organization : Nippon Dental University

Other organizations : Prefectural University of Hiroshima

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of IL-6 in OP9 cells

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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