Histological Evaluation of Bone Healing

The structural and cellular changes during the progression of healing were investigated using the following histological stains: (1) Trichrome Masson Goldner stain [90 (link),91 (link)] to obtain an overview of mineralized and nonmineralized bone matrix changes within the fractured callus. (2) Sirius red stain [65 (link)] to investigate the changes in type I collagen distribution with the progression of healing. The stain facilitates the visualization of type I and type III collagen under polarized light. However, this study mainly focused on investigating the organization of type 1 collagen using Sirius red stain and silver nitrate stain [52 (link),91 (link)] to examine the morphological changes of osteocytes over the course of healing.

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Malhan D., Schmidt-Bleek K., Duda G.N, & El Khassawna T. (2023). Landscape of Well-Coordinated Fracture Healing in a Mouse Model Using Molecular and Cellular Analysis. International Journal of Molecular Sciences, 24(4), 3569.

Publication 2023

Bone matrix Callus Cellular Light Osteocytes Progression Silver nitrate stain Stain Trichrome stain Type 1 collagen Type iii collagen

Corresponding Organization : University of Giessen

Other organizations : MSH Medical School Hamburg – University of Applied Sciences and Medical University, Berlin Institute of Health at Charité - Universitätsmedizin Berlin

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Variable analysis

independent variables

Progression of healing over time

dependent variables

Structural and cellular changes within the fractured callus
Changes in type I collagen distribution
Morphological changes of osteocytes

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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