All chemicals, including Protease Type XIII from Aspergillus saitoi (≥0.6 unit/mg) and deuterium oxide (≥99.6 atom % D), were purchased from Sigma-Aldrich (Milwaukee, WI, USA) unless noted otherwise. An ACE® Excel® SuperC18™ column (100 mm × 2.1 mm, 1.7 μm, 90 Å) was purchased from Advanced Chromatography Technologies Ltd. (Aberdeen, Scotland). Recombinant PA was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). Fully human monoclonal antibodies p6C01, p1C03, p1A06, and p6C04 were produced in HEK293 cells as previously described [9 (link),19 (link)].
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