Nicotine hydrogen tartrate salt (12.6mg/kg/day freebase, Sigma, St. Louis, MO) dissolved in 0.9% saline was administered subcutaneously through osmotic mini-pumps (Alzet, Model 1002, Durect, Cupertino, CA). Pre-adolescent conditioned mice were PND 25 and adult conditioned mice were PND 55 at the time of surgery. Mini-pumps were surgically implanted through an incision on the upper back of the mouse while mice were under 2–5% isoflurane anesthetic. Control mice received a mini-pump containing only saline. Surgeries were performed under aseptic conditions. Nicotine dosage was chosen based on the previous findings showing deficits in contextual fear learning during extinction testing at this dose (Kutlu et al., 2016 (link)).
Protocol detail
Nicotine Infusion for Contextual Fear Learning
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0 9 saline
Adolescent
Adult
Anesthetic
Aseptic
Extinction
Fear
Hydrogen
Isoflurane
Mice
Nicotine
Nicotine tartrate
Osmotic
Saline
Salt
Surgeries
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Variable analysis
independent variables
- Nicotine hydrogen tartrate salt (12.6mg/kg/day freebase, Sigma, St. Louis, MO) dissolved in 0.9% saline
- Contextual fear learning during extinction testing
- Age of mice (pre-adolescent PND 25 and adult PND 55)
- Surgical implantation of osmotic mini-pumps under 2-5% isoflurane anesthetic
- Aseptic surgical conditions
- Mini-pump containing only saline
- Not explicitly mentioned
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