HCT 116 colon adenocarcinoma cell line (p53+/+) were obtained from ATCC (Manassas, VA); HCT 116 p53−/− cells were kindly provided by Dr. Bert Vogelstein [10 (link), 11 (link)]. Cells were cultured in RPMI-1640 medium with L-glutamine (BioWhittaker Inc., Walkersville, MD), containing 10% heat-inactivated fetal bovine serum and 100 units of penicillin/mL and 100 µg/mL of streptomycin (Biofluids, BioSource, Rockville, MD) in an incubator with 95% air, 5% CO2, and 95% humidity at 37°C.
Protocol detail
Cultivation of Isogenic Colon Cancer Cell Lines
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HCT 116 colon adenocarcinoma cell line (
HCT 116
RPMI-1640 medium with L-glutamine
penicillin
streptomycin
Cells
Colon adenocarcinoma
Cultured medium
Fetal bovine serum
Hct 116 cells
Humidity
L glutamine
Penicillin
Streptomycin
Corresponding Organization :
Other organizations : UPMC Hillman Cancer Center, University of Pittsburgh
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Variable analysis
independent variables
- Cell line (HCT 116 colon adenocarcinoma cell line (p53+/+) and HCT 116 p53-/- cells)
- Not explicitly mentioned
- Culture medium (RPMI-1640 medium with L-glutamine)
- Serum (10% heat-inactivated fetal bovine serum)
- Antibiotics (100 units of penicillin/mL and 100 µg/mL of streptomycin)
- Incubator conditions (95% air, 5% CO2, 95% humidity at 37°C)
- Positive control: HCT 116 colon adenocarcinoma cell line (p53+/+)
- Negative control: HCT 116 p53-/- cells
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