The S. suis reference strains 10 and A3286/94 were used in this study and are referred to as serotype 2 and 9 strain, respectively. Strain 10 is an MRP+ EF+ SLY+ serotype 2 strain which has been used successfully by different groups to induce disease experimentally in either intranasal or intravenous application models with piglets [34 (link),35 (link),36 (link),37 (link),38 (link),39 (link)]. The MRP* SLY+ serotype 9 S. suis strain A3286/94 caused disease after intravenous injection but only subclinical pathological findings after intranasal application [40 (link)]. Cultivation of S. suis was conducted on Columbia agar with 6% sheep blood supplemented in appropriate cases with StaphStrep selective Supplement or in Todd-Hewitt broth (THB, all Oxoid, Wesel, Germany) at 37 °C for 24 h.
The Salmonella enterica ssp. enterica Serovar Derby (Salmonella Derby) strain A147/85 (O 1,4, 12; H:f,g) was cultivated on Columbia agar with 6% sheep blood, on BrillianceTMSalmonella agar, on Brilliant Green agar (all Oxoid, Wesel, Germany) or in lysogeny broth as appropriate.
The Salmonella enterica ssp. enterica Serovar Derby (Salmonella Derby) strain A147/85 (O 1,4, 12; H:f,g) was cultivated on Columbia agar with 6% sheep blood, on BrillianceTMSalmonella agar, on Brilliant Green agar (all Oxoid, Wesel, Germany) or in lysogeny broth as appropriate.
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