Flow cytometry was performed to examine the proportions of STRO-1+CD117+ cells in U2OS and 143B cells as previous described [18 (link)]. U2OS and 143B cells were cultured in DMEM medium, and the cell suspension (106 cells/100 μL) was incubated with 10 μL STRO-1-PE or CD117-APC (Abcam) at 4°C for 30 min in darkness. After that, the proportions of STRO-1+CD117+ cells in U2OS and 143B cells were detected using a BD FACScaliber flow cytometer (BD Biosciences, San Jose, CA, USA). The fluorescent intensity was analyzed using Cell Quest Software (BD Biosciences).
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Quantifying STRO-1+CD117+ Cells in Osteosarcoma
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Corresponding Organization : Wuhan University
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Variable analysis
independent variables
- Cell type (U2OS and 143B cells)
- Proportions of STRO-1+CD117+ cells
- Culture medium (DMEM)
- Cell suspension concentration (10^6 cells/100 μL)
- Incubation conditions (4°C, 30 min, darkness)
- Flow cytometry equipment (BD FACScaliber flow cytometer)
- Analysis software (Cell Quest Software)
- No positive or negative controls were explicitly mentioned in the provided information.
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