Protein Expression Analysis by SDS-PAGE and Western Blot

Sodiumdodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting were performed as described previously 5 (link). The following antibodies were used: MAD2 (BD BioScience, San Jose, CA, USA), HDAC6 (Millipore, Billerica, MA, USA), Caspase 3, p21, poly (ADP-ribose) polymerase (PARP), CDK6 and p-Rb (Ser 807/811) (Cell Signalling, Danvers, MA, USA), β-actin and p16 (Santa Cruz Biotechnology, Dallas, Texas, USA), beta (β)-galactosidase (Abcam, Cambridge, UK).

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Weiner-Gorzel K., Dempsey E., Milewska M., McGoldrick A., Toh V., Walsh A., Lindsay S., Gubbins L., Cannon A., Sharpe D., O'Sullivan J., Murphy M., Madden S.F., Kell M., McCann A, & Furlong F. (2015). Overexpression of the microRNA miR-433 promotes resistance to paclitaxel through the induction of cellular senescence in ovarian cancer cells. Cancer Medicine, 4(5), 745-758.

Publication 2015

HDAC6

Actin Antibodies Caspase 3 Cdk6 Poly adp ribose polymerase Polyacrylamide gel electrophoresis Sulphate β galactosidase

Corresponding Organization :

Other organizations : University College Dublin, St. James's Hospital, Queen's University Belfast, Dublin City University, Mater Misericordiae University Hospital

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Variable analysis

independent variables

Treatment or experimental condition (not explicitly mentioned)

dependent variables

Protein expression levels of MAD2, HDAC6, Caspase 3, p21, PARP, CDK6, p-Rb (Ser 807/811), β-actin, and p16
β-galactosidase activity

control variables

Experimental procedures for SDS-PAGE and Western blotting were kept consistent across samples, as described in the previous publication (link provided)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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