Primary HASMC were obtained from Innoprot (Derio, Spain) and cultured as described.10 (link) Confluent cells were treated with medium supplemented with 20% of control or uremic human serum for the indicated period of time. When applicable, the Kv1.3-specific inhibitors 5-(4-phenoxybutoxy) psoralen (PAP-1, Sigma-Aldrich) and margatoxin (MgTx) were used at 100 nM22 (link),23 (link) and 10 nM,24 (link) respectively. All experiments were performed using cells between Passages 3 and 5.
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Vascular Smooth Muscle Cell Serum Response
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Corresponding Organization : Universidad de Valladolid
Other organizations : Hospital Universitario Nuestra Señora de Candelaria, Centre de Recherche des Cordeliers, Inserm
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Variable analysis
independent variables
- Control or uremic human serum (20% concentration)
- Kv1.3-specific inhibitors PAP-1 (100 nM) and margatoxin (10 nM)
- Not explicitly mentioned
- Cell passages (between 3 and 5)
- Control human serum
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