Synthesis, Characterization, and Applications

1-Azidoundecane-11-thiol and Hemin-yne were synthesized following the reported procedure29 (link)69 (link). 6-Mercaptohexanoic acid was purchased from Sigma Aldrich. Di-sodium hydrogenphosphate dihydrate (Na₂HPO₄. 2H₂O) was purchased from Merck. 2, 6-lutidine was purchased from Avra Synthesis Pvt. Ltd. These chemicals were used without further purification. Au wafers were purchased from Platypus Technologies (1,000 Å of Au on 50 Å of Ti adhesion layer on top of a Si(III) surface). Transparent Au wafers (100 Å of Au on 10 Å of Ti) were purchased from Phasis, Switzerland. Au and Ag discs for the RRDE and SERRS experiments, respectively, were purchased from Pine Instruments, USA. The Mb mutants were prepared as reported in the literature7 (link)30 . Analysis of the components of the rR spectrum was done by using Lorenztian line shape of peak fit software.