Genome Sequencing of Streptococcus suis Strain WUSS351

A Bacterial DNA Kit (OMEGA Biotech, Norcross, GA, USA) was used to extract the genomic DNA from S. suis strain WUSS351, following the manufacturer’s guidelines. Novogene Bioinformatics Technology Co., Ltd (Beijing, China) used the Illumina NovaSeq PE150 system (Illumina, San Diego, CA, USA) to sequence the genomic DNA. SOAP denovo, version 2.04 [25 (link)] was used to carry out the de novo genome assembly. We deposited the genome sequence and annotation for strain WUSS351 at NCBI (Accession No. NZ_CP039462.1). Possible genomic islands (GIs) in the genome were predicted using Island Viewer 4 [26 (link)]. BLAST algorithms at the NCBI were used to perform homology analysis. The genetic distance among S. suis strains was calculated using the OrthoANIu algorithm [27 (link)]. Based on the distance data, a phylogenetic tree by the Neighbor-Joining method was generated with MEGA X software [28 (link)]. Multiple alignments of genomes were carried out using the Mauve software [29 (link)], and the Circos software was used to display the comparison results [30 (link)].