Optimized Organ Preservation Solution

A modified Histidine-Tryptophan-Ketoglutarate (HTK) organ preservation solution was prepared in house with 5% (w/v) polyethylene glycol (PEG), average M.W. 8000, added as an oncotic agent to minimize cellular edema [6 (link)–9 (link)]. The modified HTK-PEG solution was sterilized by filtration through a 0.22 μm filter unit, and the pH and density was measured. The density of the HTK-PEG preservation solution was measured using a Densito 30PX densitometer (Mettler Toledo) approximately 1.022 g/mL at 20 °C. The solution pH was 7.2-7.4 at 4 °C with a theoretical osmolality of approximately 315 mOsm/kg.