VRK1 Depletion Across Cell Lines

The depletion of VRK1 by siRNA has been previously reported for A549 [20 (link), 23 (link)], H1299 [20 (link), 23 (link)], MDA-MB-231 [26 (link)] and HT144 [23 (link), 24 (link)] cell lines. Specific VRK1 knockdown was performed using siVRK1–02 from DHARMACON RNA Technologies. The target sequences of this siVRK1–02 is the following one (5′ to 3′): CAAGGAACCUGGUGUUGAA. The “ON-TARGETplus siCONTROL Non-targeting siRNA” from DHARMACON was used as negative control (siCtrl) [31 (link)]. Briefly, cells were transfected with the indicated siRNA at a concentration of 20 nM using either Lipofectamine 2000 (Invitrogen) or Lipotransfectin (Nivorlab), following manufacturer’s instructions [25 (link), 31 (link), 32 (link)].