Silencing Signaling Pathway Proteins

Knockdown of c-Fos and c-Jun was performed using 20 nM siRNAs from Santa Cruz (sc29221 and sc29223). Knockdown of JNK, p38K and ERK1/2 was performed using 50 nM SignalSilence^® SAPK/JNK siRNA I #6233, SignalSilence^® p38 MAPK siRNA I #6564, and SignalSilence^® p44/42 MAPK (Erk1/2) siRNA #6560. For transfection the Lipofectamine RNAiMAX Transfection Kit (Invitrogen) was used. Down-regulation of BIM was achieved by the transfection with shRNA vectors [56 (link)].

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Tomicic M.T., Meise R., Aasland D., Berte N., Kitzinger R., Krämer O.H., Kaina B, & Christmann M. (2015). Apoptosis induced by temozolomide and nimustine in glioblastoma cells is supported by JNK/c-Jun-mediated induction of the BH3-only protein BIM. Oncotarget, 6(32), 33755-33768.

Publication 2015

siRNAs Lipofectamine RNAiMAX Transfection Kit

C jun Down regulation Erk1 Lipofectamine P38 mapk Shrna Sirna Transfection Vectors

Corresponding Organization : University Medical Center of the Johannes Gutenberg University Mainz

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Variable analysis

independent variables

Knockdown of c-Fos using 20 nM siRNAs from Santa Cruz (sc29221)
Knockdown of c-Jun using 20 nM siRNAs from Santa Cruz (sc29223)
Knockdown of JNK using 50 nM SignalSilence® SAPK/JNK siRNA I #6233
Knockdown of p38K using 50 nM SignalSilence® p38 MAPK siRNA I #6564
Knockdown of ERK1/2 using 50 nM SignalSilence® p44/42 MAPK (Erk1/2) siRNA #6560
Down-regulation of BIM using shRNA vectors [56 (link)]

dependent variables

Not explicitly mentioned

control variables

Lipofectamine RNAiMAX Transfection Kit (Invitrogen) used for transfection

controls

Positive control: Not specified
Negative control: Not specified

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