Western blotting and immunofluorescence assays (IFAs) were performed as previously described (26 (link)). Immunoblots were probed with anti-Ty mouse monoclonal antibody (MAb BB2) at 1:1,500, and loading controls were extracted from Stain-Free gel (Bio-Rad) total protein images. Anti-CP1 mouse polyclonal antibody (26 (link)) was used at a dilution of 1:500.
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