Sirt1 and Sirt3 siRNA Transfection in H9c2 Cells

H9c2 cells were transfected with Sirt1 siRNA (sc-108043) or control siRNA with Sirt1 siRNA Transfection Reagent (Santa Cruz) when cells proliferated to 50% to 60% confluence, according to manufacturer's instructions. Similarly, H9c2 cells were transfected with Sirt3 siRNA or control siRNA with Lipofectamine 2000 (Invitrogen). The sense sequences of Sirt3 siRNA was as follows: 5′-UUC UCC GAA CGU GUC ACG UTT-3′ (Genepharma, Shanghai, China) (10 (link)).

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Huang D.D., Wei X.H., Mu H.N., Pan C.S., Li Q., Hu B.H., Chang X., Yan L., Fan J.Y., Liu Y.Y., Luo J.Y, & Han J.Y. (2019). Total Salvianolic Acid Injection Prevents Ischemia/Reperfusion-Induced Myocardial Injury Via Antioxidant Mechanism Involving Mitochondrial Respiratory Chain Through the Upregulation of Sirtuin1 and Sirtuin3. Shock (Augusta, Ga.), 51(6), 745-756.

Publication 2019

Lipofectamine 2000 Sirt3 siRNA

Cells Lipofectamine 2000 Sirna Sirt1 Sirt3 Transfection

Corresponding Organization : Shanghai Innovative Research Center of Traditional Chinese Medicine

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Variable analysis

independent variables

Sirt1 siRNA (sc-108043)
Control siRNA
Sirt3 siRNA
Control siRNA

dependent variables

Not explicitly mentioned

control variables

Cell proliferation to 50% to 60% confluence
Sirt1 siRNA Transfection Reagent (Santa Cruz)
Lipofectamine 2000 (Invitrogen)

controls

Positive control: Transfection with control siRNA
Negative control: Not explicitly mentioned

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