Fungal Biomass Extraction and Bacterial Culture

Each fungal strain was inoculated into 200 mL potato dextrose broth (PDB) medium at 200 rpm on a rotary shaker at 28°C for 7–10 days. The fungal broth was filtered using Whatman No. 5 filter paper, and then the protein in filtrate was removed by the sevage method (Kusaikin et al., 2010 (link)). The mycelia was dried and then extracted twice with 95% ethyl alcohol (1:2/V:V) by circumfluence extraction (Cui et al., 2015 (link)). The bacteria was inoculated in beef extract-peptone medium at 200 rpm on a rotary shaker at 37°C for 48–72 h. All the extracting solution was concentrated in a rotary evaporator at 60°C and further was removed by evaporation under 40°C.