Hi-C Chromosome-level Genome Assembly
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Corresponding Organization : Shenyang Agricultural University
Other organizations : Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Variable analysis
- About 1.5 g of 3-week-old seedlings were used for the Hi-C library construction.
- Hi-C libraries were created and sequenced on the Illumina X-TEN platform.
- Mapping of Hi-C reads and assignment to restriction fragments were performed.
- The raw counts of the Hi-C links were aggregated in 100-kb bins and normalized separately for intra- and inter-chromosomal contacts.
- The corrected contigs were assembled into 12 chromosome-level scaffolds.
- Adjacent contigs were linked together by filling the gap with 'N'.
- 12 high-quality pseudochromosome-level genomes were built for representative GJ accessions.
- Not explicitly mentioned.
- Not specified.
- Not specified.
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