Placental histocultures and infection were carried out as described [25 (link),37 (link)] on first trimester placentas (4 placentas; mean = 13.11 ± 0.49 (SEM) weeks of amenorrhea, i.e., 11.11 ± 0.49 weeks of pregnancy; age of the women: mean = 23 ± 1.5 (SEM) year-old). Briefly, trophoblastic villi were dissected in small explants and infected or not by hCMV overnight before extensive washing and deposition on gelatin sponges (Gelfoam, Pfizer, New York, NY, USA) in Exofree medium. Conditioned medium was collected and renewed every 3 to 4 days. At 14 days of culture, collected medium was pooled for each condition and used to perform sEV preparation. Placental explants were weighed for normalization of resuspension volume.
Bergamelli M., Martin H., Aubert Y., Mansuy J.M., Marcellin M., Burlet-Schiltz O., Hurbain I., Raposo G., Izopet J., Fournier T., Benchoua A., Bénard M., Groussolles M., Cartron G., Tanguy Le Gac Y., Moinard N., D’Angelo G, & Malnou C.E. (2022). Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro. Viruses, 14(9), 2030.
Corresponding Organization : Centre National de la Recherche Scientifique
Other organizations :
Hôpital Purpan, Institut National Polytechnique de Toulouse, Institut de Pharmacologie et de Biologie Structurale, Institut Curie, Université Paris Cité, Association Francaise contre les Myopathies, Institut des Cellules Souches pour le Traitement et l'Étude des Maladies Monogéniques, Hôpital des Enfants, Hôpital Paule de Viguier, Laboratoire Epidémiologie et Analyses en Santé Publique : Risques, Maladies Chroniques et Handicaps, Université de Montpellier
First trimester placentas (4 placentas; mean = 13.11 ± 0.49 (SEM) weeks of amenorrhea, i.e., 11.11 ± 0.49 weeks of pregnancy; age of the women: mean = 23 ± 1.5 (SEM) year-old)
Trophoblastic villi dissected in small explants and deposited on gelatin sponges (Gelfoam, Pfizer, New York, NY, USA) in Exofree medium
Conditioned medium collected and renewed every 3 to 4 days
Placental explants weighed for normalization of resuspension volume
positive control
Not explicitly mentioned
negative control
Not explicitly mentioned
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