Comprehensive Western Blot Analysis

Western blot assay was conducted as previously described [24 (link)]. Antibodies used in this study were as follows: anti-GAPDH (10494-1-AP, Proteintech), anti-AKT (C67E7, Cell Signaling Technology), anti-pAKT (S473) (193H12, Cell Signaling Technology), anti-PI3K (Y388, Abcam), anti-PI3K (p110) (Y384, Abcam), anti-S1PR3 (EPR454, Abcam), anti-EGR2 (EPR4004, Abcam), anti-IGF2BP1 (22803-1-AP, Proteintech), anti-IGF2BP2 (11601-1-AP, Proteintech), anti-IGF2BP3 (14642-1-AP, Proteintech), anti-WTAP (10200-1-AP, Proteintech).

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Ying Y., Ma X., Fang J., Chen S., Wang W., Li J., Xie H., Wu J., Xie B., Liu B., Wang X., Zheng X, & Xie L. (2021). EGR2-mediated regulation of m6A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization. Cell Death & Disease, 12(8), 750.

Publication 2021

anti-GAPDH (10494-1-AP, anti-AKT (C67E7, anti-pAKT (S473) anti-PI3K (p110) ( EPR4004, anti-IGF2BP1 (22803-1-AP, anti-IGF2BP2 ( anti-IGF2BP3 ( anti-WTAP (

Antibodies Egr2 Gapdh Igf2bp2 Igf2bp3 Pi3k Western blot assay

Corresponding Organization :

Other organizations : Zhejiang University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of GAPDH, AKT, pAKT (S473), PI3K (Y388), PI3K (p110), S1PR3, EGR2, IGF2BP1, IGF2BP2, IGF2BP3, WTAP

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

Annotations

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