Human tumour cell lines 22Rv1, RT4, DU145 and T24 were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Meilunbio, Dalian, China) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, MA, USA) and a cocktail of antibiotics (Beyotime Biotechnology, Shanghai, China). MCF7, T47D, Huh7 and HCT116 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Meilunbio, Dalian, China) containing 10% FBS, 100 U/mL penicillin and 100 mg/mL streptomycin. All cells were purchased from ATCC and cultured in a humidified incubator at 37 °C with 5% CO2.
The MDM2 inhibitor XR-2 was designed and synthesized by Wu et al. [22 (link)]. The NMD inhibitor was synthesized according to the structure of the previous study [25 (link)]. RG7388 was purchased from Selleck (Selleck Chemicals, TX, USA). All compounds were dissolved in dimethyl sulfoxide (DMSO) and stored at −20 °C until further use.
The MDM2 inhibitor XR-2 was designed and synthesized by Wu et al. [22 (link)]. The NMD inhibitor was synthesized according to the structure of the previous study [25 (link)]. RG7388 was purchased from Selleck (Selleck Chemicals, TX, USA). All compounds were dissolved in dimethyl sulfoxide (DMSO) and stored at −20 °C until further use.
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