All studies complied with the requirements of the Institutional Animal Care and Use Committee (IACUC, 19–806-B). Male CD1 mice (7–8 weeks old) (Crl:CD1(ICR)) were purchased from Charles River Laboratory (Wilmington, MA, USA). RFP-Exo and RFP-Lipo were administered via nebulization (Pari Trek S Portable 459 Compressor Nebulizer Aerosol System; 047F45-LCS, PARI). PBS, Exo, RBD, RBD-Exo treatments (1010 per kg of mouse weight) and RBD-Lipo treatments (1.26 × 1010 per kg of mouse weight) were given in two doses once a week for 2 weeks via nebulization or IV injection. Spike protein (Sino Biological) was modified onto the surface of lentivirus (Cellomics Technology) and the SARS-CoV-2 mimics were synthesized according to our previous report59 (link). Mice were challenged with SARS-CoV-2 mimics labelled with AF647 (106 particles per kg of body weight) by nebulization 1 week after the second treatment dose. Lungs were excised and imaged at days 2 and 6 post vaccination with a Xenogen live imager (PerkinElmer). Blood and all other major organs were collected for further analysis.
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SARS-CoV-2 Mimics Challenge in Mice
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Corresponding Organization :
Other organizations : North Carolina State University, Duke University, University of North Carolina at Chapel Hill
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Variable analysis
independent variables
- RFP-Exo administration via nebulization
- RFP-Lipo administration via nebulization
- PBS administration via nebulization or IV injection
- Exo administration via nebulization or IV injection
- RBD administration via nebulization or IV injection
- RBD-Exo administration via nebulization or IV injection
- RBD-Lipo administration via nebulization or IV injection
- SARS-CoV-2 mimics labelled with AF647 administration via nebulization
- Lung imaging at days 2 and 6 post vaccination
- Blood and other major organ collection for further analysis
- Male CD1 mice (7-8 weeks old) (Crl:CD1(ICR)) from Charles River Laboratory
- Compliance with Institutional Animal Care and Use Committee (IACUC, 19-806-B) requirements
- None specified
- PBS administration via nebulization or IV injection
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