Quantitative Proteomic Analysis of Exosomes
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Corresponding Organization : Daegu Gyeongbuk Institute of Science and Technology
Other organizations : Korea Brain Research Institute, University Medical Center Freiburg, University of Freiburg, National Cancer Center, Yonsei University, Pohang University of Science and Technology, National Institute on Alcohol Abuse and Alcoholism
Variable analysis
- Exosomal protein digestion with trypsin
- Tryptic peptide analysis using nano‐ultra‐high‐performance LC (UPLC) and tandem mass spectrometry
- Protein identification using MASCOT
- Protein quantification using PEAKS Studio
- All proteomic experimental procedures were performed according to a previous report (Im et al., 2019)
- Peptide injection into a 2 cm × 180 μm trap column and resolution using a 25 cm × 75 μm nano ACQUITY C18 column
- Analysis of all samples in triplicate
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