The SSU region, as well as the ITS1-5.8S-ITS2 region (hereafter, ITS-1,2 regions), and in particular groups, the ITS-2 region for green algae and the SSU-LSU rRNA (large subunit rRNA) intergenic spacer for cyanobacteria were used as molecular markers. The cells of green algae and cyanobacteria were disrupted by shaking with 1.25–1.55-mm glass beads in combination with a threefold freezing and thawing cycle (liquid N, heating block 65 °C). The DNA was extracted with the NucleoSpin Plant II mini kit (Macherey Nagel, Düren, Germany) following the instructions. PCR was performed as described by Mikhailyuk et al. [86 (link)] using the primers EAF3 and ITS055R for green algae [87 (link),88 (link)] and SSU-4-forw and ptLSU C-D-rev for cyanobacteria [89 (link)]. Sanger sequencing was conducted by GATC Sequencing Services (Eurofins Genomics Germany, Ebersberg, Germany) using the primers EAF3 and 1400R [87 (link)], N920R [88 (link)], 536R [90 ], 920F and 1400F [91 (link)], and GF and GR [92 (link)] for green algae and SSU-4-forw, Wil 6, Wil 12, Wil 14, Wil 5, Wil 9, Wil 16, and ptLSU C-D-rev [89 (link),93 (link)] for cyanobacteria.
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