Corneal Stem Cell Differentiation and Transplantation

LSCs and SESCs were isolated from rabbits and human donors in feeder-free media and differentiated in the three-dimensional culture conditions. Histology, immunohistochemistry and immunocytochemistry were carried out on paraffin sections as well as on cultured cells. Gene expression microarray, RNA-seq and quantitative PCR (qPCR) were performed using total RNA isolated from LSCs, SESCs and CECs. Lentiviral RNA interference and engineered-expression study of WNT7A, PAX6 and FZD5 were carried out in LSCs and SESCs. Cell transplantation of LSCs and SESCs was performed on animal models of corneal injury. Detailed information is provided in the supplement.
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Ouyang H., Xue Y., Lin Y., Zhang X., Xi L., Patel S., Cai H., Luo J., Zhang M., Zhang M., Yang Y., Li G., Li H., Jiang W., Yeh E., Lin J., Pei M., Zhu J., Cao G., Zhang L., Yu B., Chen S., Fu X.D., Liu Y, & Zhang K. (2014). WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis. Nature, 511(7509), 358-361.

Publication 2014

Animal models Cell transplantation Cultured cells Donors Gene expression Human Immunocytochemistry Immunohistochemistry Injury of corneal Microarray Paraffin Rabbits Rna interference Rna seq Supplement Wnt7a

Corresponding Organization :

Other organizations : University of California, San Diego, Sichuan University, Sun Yat-sen University

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Protocol cited in 16 other protocols

Variable analysis

independent variables

Isolation of LSCs and SESCs from rabbits and human donors
Differentiation of LSCs and SESCs in three-dimensional culture conditions
Lentiviral RNA interference and engineered-expression study of WNT7A, PAX6 and FZD5 in LSCs and SESCs

dependent variables

Histology, immunohistochemistry and immunocytochemistry of paraffin sections and cultured cells
Gene expression microarray, RNA-seq and quantitative PCR (qPCR) of LSCs, SESCs and CECs
Cell transplantation of LSCs and SESCs on animal models of corneal injury

control variables

Feeder-free media used for isolation and differentiation of LSCs and SESCs

controls

No positive or negative controls were explicitly mentioned in the provided information.

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