Protocol detail

Hydrophilic Metabolite Extraction and GC-MS Analysis

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The hydrophilic metabolites from the cells were extracted by modifying a previously described (Kim et al. 2017 (link)). The sample was transferred into auto-sampler vials and analyzed by gas chromatography-mass spectrometry (GC-MS, a GCMS-QP2010 Ultra system, Shimadzu, Kyoto, Japan). GC-MS analysis was performed as previously described (Song et al. 2021 (link)). Pearson's correlation analysis was carried out using SAS software 9.4 (SAS Institute, San Diego, CA, USA) and then visualized with Hierarchical Clustering Analysis (HCA) using Multi-Experiment Viewer version 4.9.0 (MeV; https://webmev.tm4.org).

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Kuk M.U., Ga Y.J., Kim Y.J., Park J.Y., Song E.S., Lee H., Lee Y.H., Ko G., Kim J.K., Yeh J.Y., Kwon H.W., Byun Y, & Park J.T. (2022). Metabolic reprogramming as a novel therapeutic target for Coxsackievirus B3. Animal Cells and Systems, 26(6), 275-282.

Publication 2022

GCMS-QP2010 Ultra system SAS software 9.4

Gc ms

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Variable analysis

independent variables

Previously described protocol for extracting hydrophilic metabolites from cells (Kim et al. 2017)

dependent variables

Composition of hydrophilic metabolites analyzed by gas chromatography-mass spectrometry (GC-MS)

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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