RK-13 and HEK293T cells were cultured essentially as described previously (59 (link), 62 (link)). mAb 58-46 (α-EU-nsp2) (14 (link)), which recognizes the N-terminal domain of nsp2, nsp2TF, and nsp2N, was a kind gift from Dr. Ying Fang. mAb-FLAG (M2) was from Sigma.
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