Karyosome Defects in Oocytes

The workflow in the second round of the screen is exactly the same as described above, except that 20–29 oocytes were examined per candidate gene. One hundred six genes showed karyosome abnormalities in 25% or more of all examined oocytes in the second round. These 106 genes were considered to show reproducible karyosome defects and therefore studied further. In all 106 genes with karyosome defects, there were two major types of karyosome defects: chromatin attachment to the nuclear envelope and chromatin distortion in the nucleus.

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Nieken K.J., O’Brien K., McDonnell A., Zhaunova L, & Ohkura H. (2023). A large-scale RNAi screen reveals that mitochondrial function is important for meiotic chromosome organization in oocytes. Chromosoma, 132(1), 1-18.

Publication 2023

Abnormalities Chromatin Genes Genes defects Nuclear envelope Nucleus Oocytes

Corresponding Organization :

Other organizations : Wellcome Centre for Cell Biology, University of Edinburgh

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Variable analysis

independent variables

Number of oocytes examined per candidate gene (20–29 oocytes)

dependent variables

Presence of karyosome abnormalities in the examined oocytes

control variables

Not explicitly mentioned

controls

Not specified

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