Gut Microbiota Alterations in DMD

In this study, we found that in mdx mice, a validated preclinical model of DMD, the disease is associated with a significant alteration in the gut microbiota composition compared with healthy controls. Along with this alteration, the plasma of mdx mice showed a reduction in the levels of gut microbiota‐related metabolites, the short‐chain fatty acids (SCFAs), and an elevation of those of endocannabinoids. Supplementation with the SCFA, sodium butyrate (NaB), rescued muscle strength and autophagy, and prevented inflammation associated with excessive endocannabinoid signaling at CB1 receptors to the same extent as deflazacort (DFZ), the standard palliative care for DMD. In C2C12 myoblasts stimulated with lipopolysaccharide, a pro‐inflammatory molecule derived from a malfunctioning gut microbiota, NaB exerted anti‐inflammatory effects, promoted autophagy, and prevented dysregulation of microRNAs that keep under negative control the CB1 receptor gene and did so in a manner depending on the activation of GPR109A and PPARγ receptors.

Free full text: Click here

Kalkan H., Pagano E., Paris D., Panza E., Cuozzo M., Moriello C., Piscitelli F., Abolghasemi A., Gazzerro E., Silvestri C., Capasso R., Motta A., Russo R., Di Marzo V, & Iannotti F.A. (2023). Targeting gut dysbiosis against inflammation and impaired autophagy in Duchenne muscular dystrophy. EMBO Molecular Medicine, 15(3), e16225.

Publication 2023

Anti inflammatory Autophagy Cb1 receptor Deflazacort Endocannabinoid Gene Gut microbiota Inflammatory Lipopolysaccharide Mdx mice Micrornas Muscle strength Myoblasts Palliative care Plasma Pparγ Short chain fatty acids Sodium butyrate

Corresponding Organization :

Other organizations : National Research Council, Federico II University Hospital, Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Max Delbrück Center, Charité - Universitätsmedizin Berlin, University of Naples Federico II

Top 5 similar protocols

Variable analysis

independent variables

Supplementation with the SCFA, sodium butyrate (NaB)

dependent variables

Muscle strength
Autophagy
Inflammation associated with excessive endocannabinoid signaling at CB1 receptors
Anti-inflammatory effects
Autophagy promotion
Dysregulation of microRNAs that keep under negative control the CB1 receptor gene

control variables

Deflazacort (DFZ), the standard palliative care for DMD
Lipopolysaccharide (a pro‐inflammatory molecule derived from a malfunctioning gut microbiota) in C2C12 myoblasts

positive controls

Deflazacort (DFZ), the standard palliative care for DMD

negative controls

C2C12 myoblasts stimulated with lipopolysaccharide (a pro‐inflammatory molecule derived from a malfunctioning gut microbiota)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!