Quantifying Hemoglobin Oxidation Kinetics

The oxidation degrees of PolyCHb sample solutions were illustrated by the MetHb content, which was measured by the published equations (Benesch et al., 1973 (link)). Calculate the hemoglobin oxidation rate constant (Kox) according to the following formula: $\mathrm{Y}=Y_{\max }\left(1-e^{-\mathrm{k}\mathrm{t}}\right)+Y_0$ Where, t represents the measurement time point, Y represents the sample’s methemoglobin content, Y_max represents the highest methemoglobin content during the experiment, Y₀ represents the initial value of methemoglobin, and K represents the sample’s oxidation rate. For oxidation reaction, the calculated K value is expressed in Koxidation, which is called K_ox for short. For the reduction reaction, the calculated K value is negative, so the reduction reaction can be understood as a “negative oxidation” reaction, and its absolute value is taken to represent the reduction rate Kreduction, or K_re for short.