Total RNA was extracted from different organs of Arabidopsis using Pure link™ RNA Mini Kit (Invitrogen, Carlsbad, CA, USA) with on-column DNase I treatment to remove residual genomic DNA. Extracted total RNA was verified for quality and quantity using a Nano-MD UV-Vis spectrophotometer (Scinco, Seoul, Korea). The complementary DNA (cDNA) was synthesized in a total 20 μL reaction volume using RevertAid Reverse transcriptase (Thermo, Waltham, MA, USA). qRT-PCR was performed using TB Green™ Premix Ex Taq™ (Takara, Shiga, Japan) and Thermal Cycle Dice real-time PCR system (Takara, Shiga, Japan), as previously reported [10 (link)]. Target gene-specific primers for the qRT-PCR analysis are listed in Table S1 .
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