Hematological parameters were assessed immediately after euthanasia using a Coulter Ac·T diff Hematology Analyzer (Beckman Coulter, Gladesville, Australia). Serum iron parameters were determined using the Iron/TIBC Reagent Kit (Pointe Scientific, Canton, MI) as previously described (Frazer et al. 2017 (link)). Tissue iron levels were assayed by colorimetric assay as previously described (Torrance and Bothwell 1968 (link)).
Free full text: Click here