SARS-CoV-2 Antigen Quantification

The SARS-CoV-2 antigen levels were determined quantitatively with the Lumipulse SARS-CoV-2 antigen test (Fujirebio, Inc., Tokyo, Japan) as previously described [12 (link)]. Briefly, 700 μL of the VTM were vortexed and centrifuged at 2000×g for 5 min. Aliquots (100 μL) of the supernatant were tested on the LUMIPULSE G600II system (Fujirebio). For samples with an antigen level > 5000 pg/mL, the samples were diluted with the kit diluent and tested again, and the antigen level was calculated based on the dilution factor. We judged the results according to the manufacturer’s instruction.

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Hirotsu Y., Maejima M., Shibusawa M., Natori Y., Nagakubo Y., Hosaka K., Sueki H., Amemiya K., Hayakawa M., Mochizuki H., Tsutsui T., Kakizaki Y., Miyashita Y, & Omata M. (2022). Direct comparison of Xpert Xpress, FilmArray Respiratory Panel, Lumipulse antigen test, and RT-qPCR in 165 nasopharyngeal swabs. BMC Infectious Diseases, 22, 221.

Publication 2022

Lumipulse SARS-CoV-2 antigen test LUMIPULSE G600II system

Antigen Dilution Sars cov 2

Corresponding Organization :

Other organizations : Yamanashi Prefectural Central Hospital

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Variable analysis

independent variables

SARS-CoV-2 antigen levels

dependent variables

SARS-CoV-2 antigen levels

control variables

Sample preparation (700 μL of the VTM were vortexed and centrifuged at 2000×g for 5 min)
Aliquot volume (100 μL of the supernatant were tested)
Dilution of samples with antigen level > 5000 pg/mL using the kit diluent

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