Serum Biomarkers Profiling Protocol

All analyses were performed at the CBCD laboratory, KSU, Riyadh, KSA. Lipid profile and fasting glucose levels were measured by the chemical analyzer (Konelab, Espoo, Finland). Serum 25(OH)D was measured with a Roche Elecsys modular analytics Cobas e411 using an electro-chemiluminescence immunoassay (Roche Diagnostics, GmbH, Mannheim, Germany). CBCD laboratory is participating in the Vitamin D External Quality Assessment Scheme (DEQAS) and quality assurance standards are according to ISO-17025. Vitamin D binding protein (VDBP), sclerostin (SOST) and all the other biochemical parameters were measured by following the protocol and instructions mentioned in our previous paper [23 (link)].

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Ansari M.G., Mohammed A.K., Wani K.A., Hussain S.D., Alnaami A.M., Abdi S., Aljohani N.J, & Al-Daghri N.M. (2021). Vitamin D Receptor Gene Variants Susceptible to Osteoporosis in Arab Post-Menopausal Women. Current Issues in Molecular Biology, 43(3), 1325-1334.

Publication 2021

Elecsys modular analytics Cobas e411

Chemiluminescence immunoassay Diagnostics Glucose Lipid Serum Vitamin d Vitamin d binding protein

Corresponding Organization : King Saud University

Other organizations : University of Sharjah, King Fahd Medical City

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Lipid profile
Fasting glucose levels
Serum 25(OH)D
Vitamin D binding protein (VDBP)
Sclerostin (SOST)
Other biochemical parameters

control variables

CBCD laboratory is participating in the Vitamin D External Quality Assessment Scheme (DEQAS) and quality assurance standards are according to ISO-17025.

controls

Positive control: Not specified
Negative control: Not specified

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