Detecting Clostridium difficile Infections

Stools samples from hospitalized inpatients and ambulatory outpatients presenting to the main hospital or UMHS off-site facilities were obtained, stored in Cary-Blair transport media and tested for C. difficile as described.20 (link) Specifically, clinician-ordered stool specimens were tested for the presence of C. difficile glutamate dehydrogenase antigen and toxins A and B using the C. DIFFICILE QUIK CHEK COMPLETE^®test (Techlab, Inc., Blacksburg, VA, USA). All antigen/toxin discordant stool tests were subjected to analysis for the tcdB gene by real-time polymerase chain reaction (PCR) (BD GeneOhm™ Cdiff Assay; Franklin Lakes, NJ, USA). C. difficile presence was confirmed by growth on taurocholate-cycloserine cefoxitin fructose agar plates and brain heart infusion-supplemented tubes at 37°C as described.21 (link) Remaining stool samples were aliquoted and stored at −80°C.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Stokely J.N., Niendorf S., Taube S., Hoehne M., Young V.B., Rogers M.A, & Wobus C.E. (2016). Prevalence of human norovirus and Clostridium difficile coinfections in adult hospitalized patients. Clinical Epidemiology, 8, 253-260.

Publication 2016

BD GeneOhm™ Cdiff Assay

Agar Antigen Assay Brain Cefoxitin Cycloserine Fructose Gene Glutamate dehydrogenase Heart Inpatients Outpatients Real time polymerase chain reaction Stool Stool tests Taurocholate Tcdb Toxin

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor, Robert Koch Institute, Office of Infectious Diseases

Top 5 similar protocols

Variable analysis

independent variables

Stool samples from hospitalized inpatients and ambulatory outpatients

dependent variables

Presence of C. difficile glutamate dehydrogenase antigen and toxins A and B
Presence of the tcdB gene by real-time polymerase chain reaction (PCR)
Growth of C. difficile on taurocholate-cycloserine cefoxitin fructose agar plates and brain heart infusion-supplemented tubes

control variables

Cary-Blair transport media used for storing stool samples
Comparison of antigen/toxin discordant stool tests to tcdB gene analysis by real-time PCR
Incubation of stool samples at 37°C

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!