After written consent was obtained from all study participants, a structured
questionnaire adapted from a previous study conducted in Kenya27 and Ethiopia17 (link) was used to collect the socio-demographic, behavioral, environmental, and
treatment-related characteristics. The questions were presented to the study
participants by face-to-face interview (Supplement material ).
Participants were instructed to bring a sputum specimen in a clean, sterile, and
screw-cupped container. The sputum specimens were examined using GeneXpert (Cepheid).28 Briefly, 1 mL of sputum was mixed with 2 mL of a buffered reagent to
liquefy the sputum and incubated at room temperature for 10 min. After
incubation, 2 mL was transferred to a cartridge in order to release the target
DNA, and then the cartridge was loaded into GeneXpert (Cepheid) machines. The
result was displayed on the computer screen after 2 h of processing.
questionnaire adapted from a previous study conducted in Kenya27 and Ethiopia17 (link) was used to collect the socio-demographic, behavioral, environmental, and
treatment-related characteristics. The questions were presented to the study
participants by face-to-face interview (
Participants were instructed to bring a sputum specimen in a clean, sterile, and
screw-cupped container. The sputum specimens were examined using GeneXpert (Cepheid).28 Briefly, 1 mL of sputum was mixed with 2 mL of a buffered reagent to
liquefy the sputum and incubated at room temperature for 10 min. After
incubation, 2 mL was transferred to a cartridge in order to release the target
DNA, and then the cartridge was loaded into GeneXpert (Cepheid) machines. The
result was displayed on the computer screen after 2 h of processing.
