CpG methylation in the promoter region of CDKN2B was quantified using pyrosequencing. The EpiTect Bisulfite Kit (Qiagen, Hilden, Germany) was used for bisulfite conversion of genomic DNA; the PyroMark Gold Q96 Reagents (Qiagen) and the PyroMarkTM Q96 ID instrument (Biotage AB, Uppsala, Sweden) were used for pyrosequencing, according to the manufacturer's instructions. A total of seven CpG sites were analyzed per sample. The primers for the analysis of CDKN2B-specific CpG regions were the same as those used by our group in a previous study [20 (link)].
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CpG Methylation Analysis of CDKN2B
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Other organizations : Seoul St. Mary's Hospital, Catholic University of Korea
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Variable analysis
independent variables
- CpG methylation in the promoter region of CDKN2B
- Quantification of CpG methylation using pyrosequencing
- Bisulfite conversion of genomic DNA using the EpiTect Bisulfite Kit (Qiagen)
- Pyrosequencing using the PyroMark Gold Q96 Reagents (Qiagen) and the PyroMarkTM Q96 ID instrument (Biotage AB)
- Analysis of seven CpG sites per sample
- Primers for the analysis of CDKN2B-specific CpG regions were the same as those used in a previous study [20]
- No positive or negative controls were explicitly mentioned in the provided information.
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