Docosahexaenoic acid (DHA) was obtained from Cayman Chemical (Ann Harbor, MI, USA), RPMI and DMEM media and fetal bovine serum (FBS) from Invitrogen (Carlsbad, CA, USA), and mouse interleukin 4 (IL-4) from Sigma Chemical (St Louis, MO, USA). Mouse diets were purchased from DYETS, Inc. (Bethlehem, PA). For the ω−6 diet, 30% of energy (134g/kg) was provided by corn oil, and the ω−6 to ω−3 ratio was 18:1. For the ω−3 diet 30% of energy was provided by menhaden oil (134g/kg) and the ω−6 to ω−3 ratio was 1:8 as previously described [11 (link)].
Protocol detail
Dietary Omega-3 and Omega-6 Fatty Acids
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Corresponding Organization : VA Greater Los Angeles Healthcare System
Other organizations : University of California, Los Angeles, University of Southern California
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Variable analysis
independent variables
- Dietary ω-6 to ω-3 ratio (18:1 vs 1:8)
- Not explicitly mentioned
- Percentage of energy provided by corn oil (30%)
- Percentage of energy provided by menhaden oil (30%)
- No positive or negative controls were explicitly mentioned in the input text.
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