Subcellular Localization Analysis of Trypanosoma Proteins

Mouse anti-TbSUMO (1C9H8) mAb (1∶2000), rabbit anti-TbRPA1 affinity-purified antiserum (1∶600) [4] (link) and rabbit anti-GFP polyclonal (1∶5000; Invitrogen) were used as primary antibodies. Goat anti-mouse and anti-rabbit Alexa 488 or 594 conjugated antibodies (Invitrogen) were used as secondary antibodies. Detailed subcellular localization and colocalization analysis was performed by deconvolution 3D microscopy as described previously [35] (link) (see Supporting Information Text S1).

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López-Farfán D., Bart J.M., Rojas-Barros D.I, & Navarro M. (2014). SUMOylation by the E3 Ligase TbSIZ1/PIAS1 Positively Regulates VSG Expression in Trypanosoma brucei. PLoS Pathogens, 10(12), e1004545.

Publication 2014

rabbit anti-GFP polyclonal Alexa 488 or 594 conjugated antibodies

Antibodies Antiserum Goat Microscopy Mouse Rabbit

Corresponding Organization :

Other organizations : Instituto de Parasitología y Biomedicina "López - Neyra", Consejo Superior de Investigaciones Científicas, Centro Nacional de Medicina Tropical, Instituto de Salud Carlos III

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Variable analysis

independent variables

Mouse anti-TbSUMO (1C9H8) mAb
Rabbit anti-TbRPA1 affinity-purified antiserum
Rabbit anti-GFP polyclonal

dependent variables

Detailed subcellular localization and colocalization analysis

control variables

Goat anti-mouse and anti-rabbit Alexa 488 or 594 conjugated antibodies

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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