Cell Apoptosis and Cell Cycle Analysis

Percentages of apoptotic cells and cell cycle phases were determined using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA) after the cells were stained with Annexin-V FITC Apoptosis Kit (Invitrogen, Carlsbad, CA) and propidium iodide/RNase (Sigma-Aldrich, St. Louis, MO), respectively, as we described recently [56 (link)]. All experiments were carried out in triplicate with separate cultures and all data were analyzed with Flowjo (Ashland, OR).

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Tu M.J., Pan Y.Z., Qiu J.X., Kim E.J, & Yu A.M. (2016). MicroRNA-1291 targets the FOXA2-AGR2 pathway to suppress pancreatic cancer cell proliferation and tumorigenesis. Oncotarget, 7(29), 45547-45561.

Publication 2016

FACSCalibur flow cytometer Annexin-V FITC Apoptosis Kit propidium iodide/RNase

Annexin v fitc Apoptosis Cell cycle Cells Propidium iodide Rnase

Corresponding Organization :

Other organizations : University of California, Davis, University at Buffalo, State University of New York, Roswell Park Comprehensive Cancer Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Percentages of apoptotic cells
Cell cycle phases

control variables

FACSCalibur flow cytometer (BD Biosciences, San Jose, CA)
Annexin-V FITC Apoptosis Kit (Invitrogen, Carlsbad, CA)
Propidium iodide/RNase (Sigma-Aldrich, St. Louis, MO)
Separate cultures for each experiment (performed in triplicate)
Flowjo (Ashland, OR) for data analysis

controls

Positive control: Not specified
Negative control: Not specified

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