A gonadotropin-releasing-hormone antagonist or gonadotropin-releasing-hormone agonist long regimen was used for ovarian stimulation in all patients. When 60% of the follicles reached 16 mm, ovulation was triggered by subcutaneous injection of 250 μg recombinant human choriogonadotropins-ping alfa solution (Merck Serono S.P.A, BA061210). Oocytes were retrieved 34 to 36 hours later and were fertilized by IVF/ICSI. Embryos were in G1-plus/G2-plus media until day 3 or day 5/6 and then transferred into the patient’s uterine cavity. Clinical pregnancy was defined as the presence of a gestational sac with yolk sac, fetal heart, and fetal bud detected by color Doppler ultrasound on the 28th day after ET.
Protocol detail
In Vitro Fertilization Protocol
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Cavity
Doppler ultrasound
Embryos
Fetal
Fetal heart
Follicles
Gestational sac
Gonadotropin releasing hormone
Human
Icsi
Oocytes
Ovarian stimulation
Ovulation
Patients
Pregnancy
Regimen
Subcutaneous injection
Uterine
Yolk sac
Corresponding Organization : Jiangxi University of Traditional Chinese Medicine
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Variable analysis
independent variables
- Gonadotropin-releasing-hormone antagonist or gonadotropin-releasing-hormone agonist long regimen for ovarian stimulation
- Follicle size (60% of follicles reached 16 mm)
- Oocyte retrieval timing (34 to 36 hours after trigger)
- Fertilization method (IVF/ICSI)
- Embryo culture media (G1-plus/G2-plus until day 3 or day 5/6)
- Embryo transfer (into patient's uterine cavity)
- Clinical pregnancy (presence of gestational sac with yolk sac, fetal heart, and fetal bud detected by color Doppler ultrasound on the 28th day after ET)
- All patients received the same ovarian stimulation protocol
- Subcutaneous injection of 250 μg recombinant human choriogonadotropins-ping alfa solution (Merck Serono S.P.A, BA061210) to trigger ovulation
- None specified
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