All animal protocols were approved by the IACUC at Baylor College of Medicine. NOD female mice were purchased from Jackson Labs (Bar Harbor, ME) at 8 weeks of age and followed weekly with non-fasting blood glucose (One touch glucometer, LifeScan) and body weight measurements between 9–10AM on regular chow (2020x Teklad). If the blood glucose was greater than 250 mg/dl, it was retested the following day. Diabetes was defined as two consecutive blood glucose measurements >250 mg/dl. Diabetic mice were treated with HDAd vectors by tail vein injection within 48 hours after the diagnosis of diabetes. Glucose tolerance testing was performed, as described before17 (link), at 4 weeks after treatment in 6 hour fasted mice with an intra-peritoneal injection of 1.5 gm/kg body weight of D-glucose. Insulin was assayed using the Mercodia Ultrasensitive ELISA kits.
Protocol detail
Glucose Tolerance Testing in Diabetic Mice
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After treatment
Animal
Blood glucose
Body
Body weight
Diabetes
Diagnosis
Elisa
Female
Glucose
Iacuc
Insulin
Intra peritoneal injection
Medicine
Mice
Nod mice
Tail
Tolerance
Touch
Vectors
Vein
Corresponding Organization :
Other organizations : Baylor College of Medicine
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Variable analysis
independent variables
- HDAd vector treatment
- Blood glucose levels
- Body weight
- Glucose tolerance
- Insulin levels
- NOD female mice
- Regular chow (2020x Teklad)
- Blood glucose measurement time (9–10AM)
- Fasting duration for glucose tolerance test (6 hours)
- None specified
- None specified
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