Immunofluorescence and Western Blot Antibody Validation

Primary antibodies used include: mouse anti-Ty (mAb BB2 [42 (link)]), rabbit HA (71–5500, ThermoFisher Scientific), mouse anti-HA (anti-HA.11, clone 16B12, Biolegend), rat anti-HA (Roche), rat anti-Flag (Biolegend), mouse mAb 45–15 anti-IMC1 (obtained from Gary Ward) [43 (link)], rabbit anti-GAP45 (obtained from Dominique Soldati-Favre) [5 (link)], mouse anti-c-myc (mAb 9E10, Life Technologies), rabbit anti-aldolase [44 (link)], rabbit anti-ROP5 [45 ], mouse mAb 6D10 anti-MIC2 [46 (link)], rabbit anti-GRA7 [47 ], rabbit anti-RON4 (obtained from John Boothroyd) [48 ] mouse mAb DG52 anti-SAG1 [49 (link)], mouse anti-ROP1 (mAb Tg49) [50 (link)], rabbit anti-MLC1 [51 (link)]. Indoleacetic acid (IAA, auxin), D-biotin and calcium ionophore A23187 were obtained from Sigma-Aldrich. Secondary antibodies used for immunofluorescence staining consisted of goat anti-mouse IgG or goat anti-rabbit IgG or goat anti-rat IgG conjugated to Alexa Fluor-488, Alexa Fluor-594 or Alexa Fluor-350 (Life Technologies). For Western blotting, secondary antibodies consisted of goat anti-mouse IgG or goat anti-rabbit IgG or goat anti-rat IgG conjugated to LiCor C800 or C680 IR-dyes and detected with an Odyssey Infrared Imaging System (LI-COR Biotechnology).

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Long S., Brown K.M., Drewry L.L., Anthony B., Phan I.Q, & Sibley L.D. (2017). Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii. PLoS Pathogens, 13(5), e1006379.

Publication 2017

rat anti-HA rat anti-Flag calcium ionophore A23187 Alexa Fluor-488 Alexa Fluor-594 Alexa Fluor-350 Odyssey Infrared Imaging System

A23187 Aldolase Alexa 594 Alexa fluor 350 Alexa fluor 488 Anti igg Antibodies Auxin Biotin Calcium ionophore Clone Dyes Goat Immunofluorescence Indoleacetic acid Mlc1 Mouse Rabbit

Corresponding Organization : Washington University in St. Louis

Other organizations : Center for Infectious Disease Research

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