For the screening of the PTK library (Selleckchem, Houston, TX) we used THP-1 cells and a 96-well plate format. All inhibitors were dissolved in DMSO and used in screening at 3 concentrations: 0.1 μM, 1 μM and 10 μM. Cells were pretreated with inhibitors for 30 min and then stimulated with 1 μg/ml of LPS (InvivoGen, San Diego, CA) for 30 min followed by 5 mM of ATP (Sigma-Aldrich) for 30 minutes. In some experiments, to further evaluate selected inhibitors, cells were infected with 100 MOI of F. novicida or 10 MOI of B. cenocepacia for 6 h. Cell culture media was collected and centrifuged for 5 minutes at 300 g. Cell death was determined by LDH release (Roche Applied Science) and inflammasome-dependent IL-18 release by ELISA, as we described earlier [8 (link)]. DMSO pre-treated cells served as a control and the AG126 PTK inhibitor (InvivoGen, San Diego, CA) served as a positive control for inflammasome inhibition [21 (link)]. To evaluate the effect of PTK inhibitors, a heat map was generated with fold difference changes related to DMSO control (100% or 1).