Unwinding Protein Interactions Quantification

All in vitro experiments were performed in at least 3 biological replicates for reproducibility of data. For quantification of unwinding, data are represented as mean ± SD. A 1-tailed paired t test was used to calculate statistical significance. P < 0.05 was considered as statistically significant. Densitometry data determined by Western blot were analyzed by PROC GLM of Statistical Analysis Systems (v9.4). F test was used to determine treatment effects, and Duncan multiple range test was used for differences between groups. Skeletal muscle fiber statistical significance was determined using 1-way ANOVA with a Tukey’s multiple-comparison post hoc test. NMJs were analyzed by a 2-way ANOVA with a Tukey’s multiple-comparison post hoc test. Analyses were all performed with GraphPad Prism software. Data are shown as mean ± SEM. Data points on graphs represent the average per animal, with statistical analysis comparing the average of each animal.