Sample Preparation Timing Across Cell Types

The time required to perform sample preparation
from the intact organ or cell culture all the way to the cell extract
depends on the number of replicates that are prepared in parallel,
the experience of the person performing the experiment, and the sample
preparation protocol at hand. We estimate the times that were required
for preparing the samples for this work as follows: peripheral blood
cells: 60 min, stem cells and progenitor cells from bone marrow: 5
hours, tissue-resident immune cells from skin: 4 hours, and splenocyte
and cultured Jurkat cells: 30 min.