Biophysical Characterization of Drug Interactions

RO3280 (≥99.5%)
and GSK461364 (≥99.8%) were supplied by MedChem Express. HSA
(≥99%; fatty acid- and globulin-free), ibuprofen (>98%),
warfarin
(99.9%), Bis-Tris (≥98.0%), NaCl (≥99.0%), and DMSO
(99.9%) were purchased from Sigma-Aldrich. The samples were dissolved
in 0.02 M Tris-HCl buffer solutions at pH 7.4 containing 0.1 M NaCl.
All of the buffer solutions were prepared in Milli Q water and filtered
with 0.22 μm filters before being used.

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Fernández-Sainz J., Pacheco-Liñán P.J., Ripoll C., González-Fuentes J., Albaladejo J., Bravo I, & Garzón-Ruiz A. (2023). Unusually High Affinity of the PLK Inhibitors RO3280 and GSK461364 to HSA and Its Possible Pharmacokinetic Implications. Molecular Pharmaceutics, 20(3), 1631-1642.

Publication 2023

HSA ibuprofen warfarin Bis-Tris NaCl DMSO

Bis tris Buffer Dmso Fatty acid Globulin Gsk461364 Ibuprofen Nacl Ro3280 Tris buffer Warfarin

Corresponding Organization : University of Castilla-La Mancha

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Variable analysis

independent variables

RO3280 (≥99.5%)
GSK461364 (≥99.8%)

dependent variables

Not explicitly mentioned

control variables

HSA (≥99%; fatty acid- and globulin-free)
Ibuprofen (>98%)
Warfarin (99.9%)
Bis-Tris (≥98.0%)
NaCl (≥99.0%)
DMSO (99.9%)
0.02 M Tris-HCl buffer solutions at pH 7.4 containing 0.1 M NaCl
Milli Q water
0.22 μm filters

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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