Conditional Knockout Mice for Intestinal Studies

B6.Cg-Tg(Vil-cre)1000Gum/J mice were purchased from the Jackson Laboratory. C57BL/6N-Sdha^{tm2a(KOMP)Wtsi} mice were obtained from the Knock Out Mouse Project (KOMP) repository, University of California, Davis and bred to ACTFLPe mice to excise the FRT-flanked region. The resulting Sdha^fl/fl mice were bred to Vil1-Cre mice to create Vil1-Cre Sdha^fl/fl (Sdha^Δ/IEC) mice (32 (link)). Then, 12 to 15 week-old mice were used in our experiments. The mice were maintained under specific pathogen-free conditions following procedures approved by the University of Michigan Committee on the Use and Care of Animals, which are based on the University of Michigan Laboratory Animal Medicine guidelines.

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Kumar R., Landry A.P., Guha A., Vitvitsky V., Lee H.J., Seike K., Reddy P., Lyssiotis C.A, & Banerjee R. (2021). A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H2S oxidation. The Journal of Biological Chemistry, 298(1), 101435.

Publication 2021

B6.Cg-Tg(Vil-cre)1000Gum/J mice

Animals Knock out mouse Laboratory animal Medicine Mice Specific pathogen free

Corresponding Organization : University of Michigan–Ann Arbor

Other organizations : Michigan Medicine

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Variable analysis

independent variables

Mouse genotype: B6.Cg-Tg(Vil-cre)1000Gum/J, C57BL/6N-Sdhatm2a(KOMP)Wtsi, Vil1-Cre Sdhafl/fl (SdhaΔ/IEC)
Mouse age: 12 to 15 weeks

dependent variables

Not explicitly mentioned

control variables

Mice were maintained under specific pathogen-free conditions
Procedures were approved by the University of Michigan Committee on the Use and Care of Animals

controls

Positive control: Not specified
Negative control: Not specified

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